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Ility Of OFFGELTM To Successfully And Reproducibly Different Milligram Quantities Of

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Ility of OFFGELTM to productively and reproducibly independent milligram quantities of protein into twelve fractionsO'Cualain et al. Malaria Journal 2010, 9:286 http://www.malariajournal.com/content/9/1/Page twelve of11. Ernoult E, Bourreau A, Gamelin E, Guette C: A proteomic technique for plasma biomarker discovery with iTRAQ labelling and OFFGEL fractionation. J Biomed Biotechnol 2010, 2010:927917. twelve. Mulvenna J, Hamilton B, Nagaraj SH, Smyth D, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/11011031 Loukas A, Gorman JJ: Proteomics Examination of the Excretory/Secretory Part from the Bloodfeeding Stage from the Hookworm, Ancylostoma caninum. Mol Cell Proteomics 2009, 8:109-121. thirteen. Nirmalan N, Sims PFG, Hyde JE: Quantitative proteomics in the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition. Mol Microbiol 2004, 52:1187-1199. 14. Examine M, Hyde JE: Simple in vitro cultivation on the malaria parasite Plasmodium falciparum (Erythrocytic phases) ideal for large-scale preparations. Methods Carbonic Anhydrase one, Human (His) Mol Biol; Protocols in molecular parasitology 1993, 21:43-55. 15. Walliker D, Beale G: Synchronization and cloning of malaria parasites. Procedures Mol Biol; Protocols in molecular parasitology 1993, 21:57-66. sixteen. Laemmli United kingdom: Cleavage of Structural Proteins Through Assembly of Head of Bacteriophage-T4. Mother nature 1970, 227:680-685. seventeen. Rabilloud T: Solubilization of proteins for electrophoretic analyses. Electrophoresis 1996, 17:813-829. eighteen. Hubner NC, Ren S, Mann M: Peptide separation with immobilized pI strips is an desirable substitute to in-gel protein digestion for proteome examination. Proteomics 2008, eight:4862-4872. 19. Apweiler R, Martin MJ, O'Donovan C, Magrane M, Alam-Faruque Y, Antunes R, Barrell D, Bely B, Bingley M, Binns D, Bower L, Browne P, Chan WM, Dimmer E, Eberhardt R, Fedotov A, Foulger R, Garavelli J, Huntley R, Jacobsen J, Kleen M, Laiho K, Leinonen R, Legge D, Lin Q, Liu WD, Luo J, Orchard S, Individual S, Poggioli D, Pruess M, Corbett M, di Martino G, Donnelly M, van Rensburg P, Bairoch A, Bougueleret L, Xenarios I, Altairac S, Auchincloss A, Argoud-Puy G, Axelsen K, Baratin D, Blatter MC, Boeckmann B, Bolleman J, Bollondi L, Boutet E, Quintaje SB, Breuza L, Bridge A, deCastro E, Ciapina L, Coral D, Coudert E, Cusin I, Delbard G, Doche M, Dornevil D, Roggli PD, Duvaud S, Estreicher A, Famiglietti L, Feuermann M, Gehant S, Farriol-Mathis N, Ferro S, Gasteiger E, Gateau A, Gerritsen V, Gos A, Gruaz-Gumowski N, Hinz U, Hulo C, Hulo N, James J, Jimenez S, Jungo F, Kappler T, Keller G, Lachaize C, LaneGuermonprez L, Langendijk-Genevaux P, Lara V, Lemercier P, Lieberherr D, Lima TD, Mangold V, Martin X, Masson P, Moinat M, Morgat A, Mottaz A, Paesano S, Pedruzzi I, Pilbout S, Pillet V, Poux S, Pozzato M, Redaschi N, Rivoire C, Roechert B, Schneider M, Sigrist C, Sonesson K, Staehli S, Stanley E, Stutz A, Sundaram S, Tognolli M, Verbregue L, Veuthey AL, Yip LN, Zuletta L, Wu C, Arighi C, Arminski L, Barker W, Chen CM, Chen YX, Hu ZZ, Huang HZ, Mazumder R, McGarvey P, Natale DA, Nchoutmboube J, Petrova N, Subramanian N, Suzek BE, Ugochukwu U, Vasudevan S, Vinayaka CR, Yeh LS, Zhang J, UniProt C: The Common Protein Resource (UniProt) in 2010. Nucleic Acids Res 2010, 38:D142-D148. 20. Malaria parasite metabolic pathways:[http://sites.huji.ac.il/malaria/]. 21. Foth BJ, Zhang N, Mok S, Preiser PR, Bozdech Z: Quantitative protein expression profiling reveals considerable post-transcriptional regulation and post-translational modifications in schizont-stage malaria par.
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